Journal: BioMed Research International

Article Title: Traditional Medicinal Plants as a Source of Antituberculosis Drugs: A System Review

doi: 10.1155/2021/9910365

Figure Lengend Snippet: Medicinal plants and natural products showing in vitro anti-TB activity.

Article Snippet: Amphipterygium adstringens , Anacardiaceae , Stem bark , Dichloromethane/methanol (1 : 1) , Tirucallanes , (14 β , 24E)-3-oxolanosta-7,24-dien-26-oic acid (16) and (14 β ,24E)-3-hydroxylanosta-7,24-dien-26-oic acid (17) with MIC of 64 and 32 μ g/ml against M . tuberculosis H37Rv (ATCC 27294) by Bactec 460-TB apparatus , [ ] .

Techniques: In Vitro, Activity Assay, Resazurin Assay, Concentration Assay, Extraction, Fluorescence, Positive Control, Inhibition, Growth Inhibition Assay, High Throughput Screening Assay, Spectrophotometry, Viability Assay

Journal: BioMed Research International

Article Title: Traditional Medicinal Plants as a Source of Antituberculosis Drugs: A System Review

doi: 10.1155/2021/9910365

Figure Lengend Snippet: Medicinal plants and their crude extracts showing in vitro anti-TB activity.

Article Snippet: Amphipterygium adstringens , Anacardiaceae , Stem bark , Dichloromethane/methanol (1 : 1) , Tirucallanes , (14 β , 24E)-3-oxolanosta-7,24-dien-26-oic acid (16) and (14 β ,24E)-3-hydroxylanosta-7,24-dien-26-oic acid (17) with MIC of 64 and 32 μ g/ml against M . tuberculosis H37Rv (ATCC 27294) by Bactec 460-TB apparatus , [ ] .

Techniques: In Vitro, Activity Assay, Inhibition, Extraction, Concentration Assay, Diffusion-based Assay

Journal: Journal of Bacteriology

Article Title: Identification and Characterization of a Regulatory Sequence Recognized by Mycobacterium tuberculosis Persistence Regulator MprA

doi: 10.1128/jb.187.1.202-212.2005

Figure Lengend Snippet: FIG. 1. Binding of mprA and pepD upstream regions by His-MprA. (A) Genetic organization of the mprAB operon and adjacent genes. DNA fragments used in electrophoretic mobility shift assays (EMSAs) were PCR amplified from M. tuberculosis H37Rv and correspond to regions immediately upstream of coding sequences for PE_PGRS18 (region 1; 211 to 50), mprAB (region 2; 240 to 37), or pepD (region 3; 164 to 50). (B) His-MprA binding to mprA and pepD upstream regions; 10 ng of DNA from regions upstream of mprA (upper) and pepD (lower) was incubated in the absence of His-MprA (lane 1), increasing amounts of His-MprA (lanes 2 to 7), or fixed amounts of His-MprA and increasing amounts of unlabeled competi- tor DNA from the same (lanes 8 to 11) or adjacent (lanes 12 to 15) region. The amount of His-MprA used in binding reactions was 6.5, 13, 26, 52, 104, and 144 pmol for lanes 2 to 7, respectively, and 20 pmol for lanes 8 to 15. Unlabeled competitor DNA from the same region was added in 0-, 10-, 100-, or 200-fold molar excess of the labeled DNA used in lanes 8 to 11, respectively, while unlabeled competitor DNA from the adjacent region was added in 0-, 100-, 200-, or 600-fold molar excess of the labeled DNA in lanes 12 to 15, respectively. (C) Phos- phorylation is not required for but enhances the binding of mprA and pepD upstream regions by His-MprA; 10 ng of DNA from the mprA or pepD upstream region was incubated in the absence (lanes 1 and 4) or

Article Snippet: The Mycobacterium strains used in this study included Mycobacterium tuberculosis H37Rv (ATCC 27294) and Mycobacterium bovis BCG Pasteur (ATCC 35734).

Techniques: Binding Assay, Electrophoretic Mobility Shift Assay, Incubation, Labeling

Journal: Journal of Bacteriology

Article Title: Identification and Characterization of a Regulatory Sequence Recognized by Mycobacterium tuberculosis Persistence Regulator MprA

doi: 10.1128/jb.187.1.202-212.2005

Figure Lengend Snippet: FIG. 2. DNase I protection assays of mprA and pepD upstream regions. DNA encompassing the mprA (A) and pepD (B) upstream regions were PCR amplified from M. tuberculosis H37Rv; 35 ng of DNA from each region was incubated with His-MprA in the presence or absence of DNase I. G, A, T, and C designate the DNA sequence ladder generated for each strand. Reactions contained no His-MprA and no DNase I (lanes 1), no His-MprA and 0.6 U of DNase I (lane 2), or 150 pmol of His-MprA and 0.6 U of DNase I (lane 3). Translational starts are indicated with arrows, and sites of DNase I hypersensitivity are indicated by *. The protected regions on each strand are indicated by black bars. (C) mprA upstream region protected by His-MprA. (D) pepD upstream region protected by His-MprA. Nucleotides comprising a conserved 8-bp direct repeat found within each protected region are highlighted and indicated with arrows.

Article Snippet: The Mycobacterium strains used in this study included Mycobacterium tuberculosis H37Rv (ATCC 27294) and Mycobacterium bovis BCG Pasteur (ATCC 35734).

Techniques: Incubation, Sequencing, Generated

Journal: Biochimica et biophysica acta. General subjects

Article Title: A new bioinspired peptide on defensin from C. annuum fruits: Antimicrobial activity, mechanisms of action and therapeutical potential.

doi: 10.1016/j.bbagen.2022.130218

Figure Lengend Snippet: Fig. 9. Effect of CaDef2.1G27-K44 on M. tuberculosis growth in bacterial culture. Bacterial suspensions (1 × 106 CFU well−1) of M. tuberculosis strain H37Rv (A) and clinical isolate M299 (B) were treated or untreated with CaDef2.1G27-K44 (200, 100, 50 and 25 μM) for 5 days. Bacterial growth in the resulted cultures was quantified by MTT test. Data are presented as a percentage of bacterial growth of treated culture compared to the growth of corresponding untreated culture (100%). Negative Control (C−) - Middlebrook 7H9 Broth supplemented with ADC; Positive Control (C+) untreated bacterial suspensions. (C) Mini mum inhibitory concentrations of CaDef2.1G27-K44 suppressing growth of the laboratory M. tuberculosis strain H37Rv and clinical M. tuberculosis isolate M299. The results presented are mean values obtained over three experiments, each done in duplicate. Dose response curves were con structed and * p < 0.05; *** p < 0.001 compared to untreated group (0 μM) determined by Tukey test.

Article Snippet: The laboratory Mycobacterium tuberculosis strain H37Rv (ATCC 27294) and highly virulent M. tuberculosis strain of the Beijing genotype, strain M299, isolated from TB patient in Mozambique and previously evaluated for virulence.

Techniques: Negative Control, Positive Control